Regular Contributions
Interleukin-13 Prevents Diaphragm
Muscle Deterioration in a Septic Animal Model
-
YUMI TAKAHASHI,
DAI KATAYOSE1 and
CHIYOHIKO SHINDOH
- Department of Medical Technology,
College of Medical Sciences, Tohoku University, Sendai 980-8575,
and 1First Department of Internal Medicine, Tohoku
University School of Medicine, Sendai 980-8574
The effects of an intravenous
injection of Interleukin-13 (IL-13) after endotoxin administration on
diaphragm muscle were studied using Wistar rats. Two treatment
groups, a control (saline+endotoxin) group and an IL-13
(IL-13+endotoxin) group were studied. E. coli endotoxin (10 mg/kg)
was injected intraperitoneally 5 minutes after saline or IL-13 (0.25
mg) injection. The force-frequency
curves, twitch kinetics and fatigability were measured at 0 and 4
hours after endotoxin injection. The force-frequency curves and
twitch tension in the control group were significantly lower at 4
hours than those at 0 hour due to endotoxin. On the other hand, IL-13
prevented the decrement of the force-frequency curves and twitch
tension induced by endotoxin. Nicotinamide adenine dinucleotide
phosphate (NADPH) diaphorase histochemistry showed positive staining
at 4 hours due to endotoxin in the control group; however, IL-13 also
blocked NADPH diaphorase staining at 4 hours. Furthermore, the
positive muscle fibers detected by the NADPH diaphorase staining were
classified as type I (slow twitch) muscle fibers by ATPase staining.
We conclude that IL-13 prevents the deterioration of contraction
induced by endotoxin by inhibiting nitric oxide production in the
diaphragm muscle, mainly the type I muscle fibers.
Key words--- endotoxin; nitric oxide; NADPH
diaphorase
© 1999 Tohoku University Medical Press
Tohoku J. Exp. Med., 1999,
189,191-202
Address for reprints: Chiyohiko Shindoh, M.D., Professor,
Department of Medical Technology, College of Medical Sciences, Tohoku
University, 2-1 Seiryomachi, Aoba-ku, Sendai 980-8575, Japan.
e-mail: cshindoh@mail.cc.tohoku.ac.jp
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