Regular Contributions
Gossypol Induced Apoptosis in the Human Promyelocytic Leukemia Cell Line HL 60
-
AYSE BALCI, FERIDE İFFET SAHİN and ABDULLAH EKMEKCİ
-
Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, 06510 Be/sevler, Ankara, Turkey
In human promyelocytic leukemia cell line HL60, apoptosis was induced by treatment with gossypol that is an inhibitor of protein kinase C. Gossypol acetic acid was added to HL 60 cells at 50, 100, 150 and 200 mM concentrations for six hours. Morphological features of apoptosis as well as internucleosomal DNA fragmentation were evaluated by light microscope, agarose gel electrophoresis and spectrofluorometric quantitation. Our results indicated that with the effective concentrations of gossypol (50 and 100 mM), apoptosis was induced in HL 60 cells.
Key words---
apoptosis; gossypol; protein kinase C
© 1999 Tohoku University Medical Press
Tohoku J. Exp. Med., 1999, 189,51-57
Address for reprints: Feride İffet Sahin, M.D., Ph.D., Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, 06510 Besevler, Ankara, Turkey.
e-mail:feridesahin@hotmail.com
Back to CONTENTS.