Gene Expression of MASH-1, MATH-1, neuroD and NSCL-2, Basic Helix-Loop-Helix Proteins, during Neural Differentiation in P19 Embryonal Carcinoma Cells
-
FUMIAKI ITOH, TOKIO NAKANE and SHIGETOSHI CHIBA
-
Department of Pharmacology, Shinshu University School of Medicine, Matsumoto 390
We examined the gene expression of MASH-1, MATH-1, neuroD and NSCL-2 during neural differentiation of P19 embryonal carcinoma cells using reverse transcription-polymerase chain reaction and high performance liquid chromatography. These proteins are members of basic helix-loop-helix transcription factor family and their expressions are reported to be transient and restricted in the nervous system during early neurogenesis. Retinoic acid (RA; 1 mM)-treatment and aggregation for 4 days induced and greatly increased MASH-1, neuroD and NSCL-2 mRNA in P19 cells. The increases peaked at day 3, 4 and 5, respectively. RA-treatment increased MATH-1 mRNA slightly. mRNA of MAP2, a neural differentiation marker, were increased by RA-treatment and the increases reached to the plateau at day 5. The results indicate that the gene expression of MASH-1, MATH-1, neuroD and NSCL-2 during neural differentiation in P19 cells is transient and the order is similar to that in the mouse embryo nervous system as previously reported.
Key words---
neural differentiation; helix-loop-helix protein; proneural gene; embryonal carcinoma cell; retinoic acid
© 1997 Tohoku University Medical Press
Tohoku J. Exp. Med., 1997, 182, 327-336
Address for reprints:
Tokio Nakane, M.D., Department of Pharmacology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390, Japan.
Back to CONTENTS.