Tohoku J. Exp. Med., 1997, 182 (2)

Decrease in Thy-1 Expression on Peripheral CD34 Positive Cells Induced by G-CSF Mobilization

SHIGERU TSUCHIYA, ATSUSHI KIKUTA1, YUKITOSHI SHIMIZU2, NAGAKUNI TAKANO3, ETSURO IT04, ARATA WATANABE5, MASUE IMAIZUMI6, TASUKE KONNO and THE TOHOKU CHILDREN LEUKEMIA STUDY GROUP

Department of Pediatric Oncology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-77, 1Department of Pediatrics, Fukushima Medical College, Fukushima 960-12, 2Department of Pediatrics, Yamagata University School of Medicine, Yamagata 990-23, 3Department of Pediatrics, Iwate Medical College, Morioka 020, 4Department of Pediatrics, Hirosaki University School of Medicine, Hirosaki 036, 5Dapartment of Pediatrics, Akita University School of Medicine, Akita 010, and 6Department of Pediatrics, Tohoku University School of Medicine, Sendai 980-77

  • In order to ascertain the cytological features of peripheral hematopoietic progenitor cells (PHPC) mobilized after administration of chemotherapeutic agents and G-CSF, lineage- and progenitor cell-specific surface markers on CD34 positive (+) cells were sequentially examined. Nineteen evaluable samples were obtained from a malignant lymphoma, an acute lymphoblastic leukemia and 5 neuroblastoma patients. CD38 and HLA-DR were respectively expressed on more than 95% and approximately 85% of CD34+ PHPC cells. CD19 was also expressed on the majority and CD117 on 10 to 20% of the CD34+ cells. The most striking finding was that the Thy-1(CDw90)+ /CD34+ population was decreased at the peak of mobilization of CD34+ cells as compared to the early phase after G-CSF administration (approximately 20% vs. 60%). These results suggest that decrease in Thy-1 expression on CD34+ cells is related to mechanisms easing CD34+ cell mobilization to the peripheral blood.
    Key words--- Thy-1; CD34; mobilization; hematopoietic progenitor cells
    © 1997 Tohoku University Medical Press

    Tohoku J. Exp. Med., 1997, 182, 157-162
    Address for reprints: Shigeru Tsuchiya, M.D., Department of Pediatric Oncology, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980-77, Japan.
    e-mail: tsuchiya@europa.idac.tohoku.ac.jp

    Back to CONTENTS.